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Biotechnology Information ncbi primer blast function
Ncbi Primer Blast Function, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+blast/blast+primer/pm42313452-86-11-9
Average 86 stars, based on 1 article reviews

ncbi primer blast function - by Bioz Stars, 2026-09

86/100 stars

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Quantitative RT-PCR:

Article Title: Disposable reagent scaffold for biochemical process integration
Article Snippet: .. After checking for specificity using Primer-BLAST (National Center for Biotechnology Information, Bethesda, MD) and self-complementarity using Multiple Primer Analyzer (ThermoFisher Scientific, Waltham, MA), the primers were evaluated via qRT-PCR using synthetic HCV 5′UTR RNA standards (Asuragen, Austin, TX). ..

Real-time Polymerase Chain Reaction:

Article Title: Dopamine D 4 receptor activation preserves morphine analgesia and attenuates tolerance by enforcing inhibitory spinal tone in rats.
Article Snippet: Prior to cDNA preparation, genomic DNA was removed by DNAse treatment (Thermo Fisher Scientific). cDNA synthesis was performed using 500 ng of total RNA per sample using the iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). .. Primers (Table 2) were designed using Primer-Blast (GeneBank, National Centre for Biotechnology Information, Bethesda, MD, USA) based on rat nucleotide sequences from the NCBI Nucleotide database, and validated with Oligoanalyzer v3.1 (Integrated DNA Technologies, Coralville, IA, USA). qPCR reactions contained cDNA equivalent to 5 ng of initial RNA, 0.5 μM final concentration of forward and reverse primers, and FastStart Essential DNA Green Master mix (Roche, Basel, Switzerland). .. Amplification was performed on a LightCycler 96 instrument (Roche) using the following protocol: 95 ◦C for 5 min, followed by 45 cycles of 95 ◦C for 10 s, 60 ◦C for 20 s, and 72 ◦C for 20 s. Product specificity was confirmed by melting curve analysis (LightCycler480, Roche) and size fractionation by 3% agarose gel electrophoresis (Supplementary Figure S2A-C).

Concentration Assay:

Gene Expression:

Article Title: An engineered triple-network alginate/gelatin hydrogel mediates osteochondral regeneration through temporal regulation of the PI3K-AKT pathway.
Article Snippet: Osteochondral defects (OCDs) present a significant clinical challenge due to the limited self-repair capacity of cartilage.. Although platelet-rich plasma (PRP) holds therapeutic potential, its efficacy is limited by the rapid release of growth factors (GFs).. In this study, we developed an injectable triple-network hydrogel, PRP@OSAGEL, composed of oxidized sodium alginate (OSA), gelatin, and PRP.

Synthesized:

Article Title: EXPRESS: Serum miR-145-5p as a Predictive Biomarker for Postoperative Pain after Percutaneous Kyphoplasty.
Article Snippet: RNA concentration and purity were assessed by NanoDrop One spectrophotometer (Thermo Fisher Scientific), with A260/A280 ratios of 1.8– 2.1 considered acceptable. .. The primer sequences were designed using Primer-BLAST (National Center for Biotechnology Information, USA) and synthesized by Shanghai Sangon Biotech Co., Ltd. (Supplementary Table 1). .. For miR-145-5p detection, U6 snRNA was used as the internal reference gene, and amplification was carried out using the Hieff® miRNA Universal qPCR SYBR Master Mix (11170ES25, Yeasen).

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